Nucleosome DNA sequence structure of isochores

DNA sequence mediates nucleosome structure and stability.

Noncoding DNA, isochores and gene expression: nucleosome formation potential

The nucleosome formation potential of introns, intergenic spacers and exons of human genes is shown here to negatively correlate with among-tissues breadth of gene expression. The nucleosome formation potential is also found to negatively correlate with the GC content of genomic sequences; the slope of regression line is steeper in exons compared with noncoding DNA (introns and intergenic space...

New and Notable DNA Sequence Mediates Nucleosome Structure and Stability

Nucleosomes form the fundamental repeating unit of eukaryotic chromatin. Subtle modifications in nucleosome structure and histone tails regulate chromatin states; hence, a comprehensive understanding of alterations in nucleosome structure is of fundamental importance in chromatin biophysics. The nature of core histone organization and nucleosome dynamics have been extensively studied using biop...

DNA Sequence Contribution to Nucleosome Distribution

DNA in eukaryotes is efficiently and compactly organized into chromatin, the fundamental subunit of which is the nucleosome: approximately 150 bp of DNA spooled 1.65 times around a histone octamer. The location and density of nucleosomes play a role in regulating nuclear processes including transcription, replication, recombination, and repair. Mechanisms acting in trans, like adenosine triphos...

The structure of DNA in a nucleosome.

We describe the application of the hydroxyl radical footprinting technique to examine the histone-DNA interactions of a nucleosome that includes part of the 5S ribosomal RNA gene of Xenopus borealis. We establish that two distinct regions of DNA with different helical periodicities exist within the nucleosome and demonstrate a change in the helical periodicity of this DNA upon nucleosome format...